The cerebral cortex plays an important role in memory, higher thinking, perception and the understanding of language. Primary Rat Cortical Neurons (RCN) are isolated from early embryonic stage (E18) Sprague-Dawley Rats and provided cryopreserved as primary cells. Post-thaw viability is consistently in the range of 50-80%. Each vial of rat cortical neurons contains at least 4 million viable cells.
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Figure 1. Long-Term Viability - Rat cortical neurons (GSC-8220) remain viable during long term culturing. Above images show cells at 1 week (A), 2 weeks (B) and 3 weeks (C) in culture.
Tips for Growing Primary Neurons in Culture Long-Term
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Figure 2. Healthy, Mature Cortical Neurons Post-thaw - Primary rat cortical neurons (GSC-8220) were isolated from Sprague-Dawley (E18) rats. MAP-2 (green) staining shows that greater than 90% of the isolated cells are neurons. DAPI staining (blue) denotes cell nuclei
Figure 3. Evoked Synaptic Release Activity - Cryopreserved and Freshly isolated primary rat neurons were each plated at 80,000 cells per well and grown for 3 weeks. All the cells in the well were stimulated simultaneously. Above data shows MTI-GlobalStem cryopreserved primary rat neurons exhibit evoked synaptic release responses virtually identical to that of fresh isolated rat forebrain neurons. The fluorescent readout was a measure of pre-synaptic release. Data courtesy of Pascal Laeng (Galenea)
A.Gene Expression Assays
Figure 4. Neuronal Transfection - Rat Cortical Neurons (GSC-8220) were transfected with GeneIn-CNS at high efficiency, while maintaining maximal cell viability.
B. Calcium Assays
Figure 5. Calcium Flux - Three week cultured rat cortical neurons (GSC-8220) labeled with Fluo-4 fluorophore show strong spontaneous synchronous calcium flux.
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