外泌體（Exosome）發(fā)現(xiàn)于1986年，是一種直徑約30~100nm的雙層膜囊泡狀結(jié)構(gòu)小體，可由機(jī)體內(nèi)多種細(xì)胞如免疫細(xì)胞、干細(xì)胞、心血管細(xì)胞、網(wǎng)織紅細(xì)胞、血小板、神經(jīng)細(xì)胞和腫瘤細(xì)胞等主動分泌產(chǎn)生，exosomes廣泛分布于外周血、尿液、唾液、乳汁、腹水、羊水等體液中。

在外泌體研究中，外泌體的分離提取是一直困擾廣大研究人員的一大難題。外泌體領(lǐng)域資深科學(xué)家Clotilde Théry所建立的超速離心提取法一直被奉為外泌體提取的“金標(biāo)準(zhǔn)”。但超速離心法由于需要樣本量大，耗時(shí)長，依賴特定儀器，提取量低，獲得外泌體純度低等缺點(diǎn)，大大降低了研究人員的研究效率。隨著外泌體研究的不斷發(fā)展，一些商品化試劑盒應(yīng)運(yùn)而生，這些試劑盒大多采用PEG沉淀原理，只需在半個(gè)小時(shí)左右的時(shí)間就可完成提取。但由于提取過程中PEG的加入，使獲得的外泌體中含有PEG偶聯(lián)物，嚴(yán)重影響后續(xù)實(shí)驗(yàn)的進(jìn)行。另外，無論是超速離心法還是PEG沉淀試劑盒，所分離的外泌體中都含有大量的白蛋白，這也給后續(xù)的實(shí)驗(yàn)造成很大的干擾。

產(chǎn)品列表

Application

Col-Tgel is a tailorable 3D cell culture matrix for tumor cell, stem cell and primary cell. We provide soft, medium and stiff gel for your different cell types.

It provides collagen backbone for cell attachment and allows customizing matrix components by additional extracellular matrix, growth factors, cytokines, and/or hormones for optimizing three dimensional cell culture.

This product is for research use only.

Col-Tgel是一種可定制的腫瘤細(xì)胞、干細(xì)胞和原代細(xì)胞三維細(xì)胞培養(yǎng)基質(zhì)。我們?yōu)槟牟煌?xì)胞類型提供軟、中、硬凝膠。

它為細(xì)胞附著提供膠原骨架，并允許通過額外的細(xì)胞外基質(zhì)、生長因子、細(xì)胞因子和/或激素定制基質(zhì)成分，以優(yōu)化三維細(xì)胞培養(yǎng)。

本產(chǎn)品僅供研究使用。

Stiffness selection for different cell types

Formation of tumor spheroids in Col-Tgel

Fang, JY., etc. PloS one 9, no. 8 (2014): e105616.

For immunohistochemistry and immunocytochemistry method, please refer to Page 2 of this publication.

Unique Features

 Handle at room temperature  (no need on ice)

·Save money:  30% below market price

·Save time: No hanging drop, rotating wall vessel bioreactors

·3 stiffness(unique feature): to fit most cell types

·2 mL aliquotes: avoid freeze-thaw cycle - user friendly

· Cytokine / Hormon free: no background

· Flexible:

-Col-Tgel can be customized for different experiments.
- Geometry:  3D embedding, 2D seeding on the top of hydrogel, or unique molds.

-   Sample size and quantity:  6- to 96- well with/without tissue culture insert or transwell.
-   Matrix component and stiffness:  collagen-based with different yield strength (i.e. Soft, Med and Stiff).
-   Transparency:  easy to observe directly under microscopes and can apply with immunohistochemistry directly.

Col-Tgel is a tailorable collagen-based, cell remodelable hydrogel system. It not only can be used a carrier for cell or drug delivery, but also in vitro cell culture platforms. Besides, it contains cell essential nutrients, and provides a stable and durable platform for long-term studies such as cell-cell, cell-matrix interactions, and etc.

Shipping / Storage

Ship at room temperature. Store Component A (gel) at 4oC, and store Component B (crosslinker) at -80oC for 9 months shelf life, or at -20oC for 3 months, or at 4oC for 1 month. Avoid freeze-thaw cycle for Component B.

Shelf Life

Component A: 12 months at 4oC

Component B: 9 months at -80oC; 3 months at -20oC; 1 month at 4oC

Citations

Citation 1:

Kuwahara, Kenrick, Zhi Yang, Ginger C. Slack, Marcel E. Nimni and Bo Han. Cell delivery using an injectable and adhesive transglutaminase–gelatin gel. Tissue Engineering Part C: Methods 16, no. 4 (2009): 609-618.

Citation 2:

Kuwahara, Kenrick, Josephine Y. Fang, Zhi Yang and Bo Han. Enzymatic crosslinking and degradation of gelatin as a switch for bone morphogenetic protein-2 activity. Tissue Engineering Part A 17, no. 23-24 (2011): 2955-2964.

Citation 3:

Fang, Josephine, Zhi Yang, ShihJye Tan, Charisse Tayag, Marcel E. Nimni, Mark Urata and Bo Han. Injectable gel graft for bone defect repair. Regenerative medicine 9, (2014): 41-51.

Citation 4:

Tan, ShihJye, Josephine Y. Fang, Zhi Yang, Marcel E. Nimni and Bo Han. The synergetic effect of hydrogel stiffness and growth factor on osteogenic differentiation. Biomaterials 35, no. 20 (2014): 5294-5306.

Citation 5:

Fang, Josephine Y., Shih-Jye Tan, Zhi Yang, Charisse Tayag and Bo Han. Tumor Bioengineering Using a Transglutaminase Crosslinked Hydrogel. PloS one 9, no. 8 (2014): e105616.

Citation 6:

Shih-Jye Tan, Fang, Josephine Y., Y. Wu, Zhi Yang, G. Liang and Bo Han. Muscle tissue engineering and regeneration through epigenetic reprogramming and scaffold manipulation. Sci Rep. 2015 Nov 9;5:16333. doi: 10.1038/srep16333.

Citation 7:

Lee G, Kim HJ, Kim HM. RhoA-JNK Regulates the E-Cadherin Junctions of Human Gingival Epithelial Cells. J Dent Res. 2015 Dec 3. pii: 0022034515619375. [Epub ahead of print]

Citation 8:

Fang JY, Tan SJ, Wu YC, Yang Z, Hoang BX, Han B. From competency to dormancy: a 3D model to study cancer cells and drug responsiveness. J Transl Med. 2016 Feb 4;14(1):38. doi: 10.1186/s12967-016-0798-8.

* Different names (TGase gel, TG gel) were used in old publications. They referred to the same 3D cell culture gel, and we finally name it as Col-Tgel.

See all 101Bio's citation papers, click here

默瑞生物

默瑞(上海)生物科技有限公司，作為專業(yè)的生命科學(xué)產(chǎn)品推廣與服務(wù)商，以“創(chuàng)新，專業(yè)，共贏”的理念，不斷強(qiáng)化服務(wù)體系，提升客戶體驗(yàn)，結(jié)合國內(nèi)外優(yōu)質(zhì)產(chǎn)品提供高標(biāo)準(zhǔn)完整解決方案，幫助科研客戶和生物技術(shù)企業(yè)高效創(chuàng)新，加速轉(zhuǎn)化，創(chuàng)造更多優(yōu)質(zhì)產(chǎn)品，提升社會福祉，實(shí)現(xiàn)多方共贏。

默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導(dǎo)入TwistDx,enzymatics,KAPA BIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,Lee Biosolutions, magtivio,美的醫(yī)療冰箱,FOUR E'S科學(xué)儀器等優(yōu)質(zhì)國際品牌，逐步培育成為專業(yè)用戶的選擇。

代理試劑產(chǎn)品有：TwistDx恒溫?cái)U(kuò)增試劑盒,Echelon脂類研究試劑,KAPA BIOSYSTEMS二代測序試劑, Enzymatics二代測序酶,Jackson ImmunoResearch抗體,ChromoTek熒光蛋白及抗體,Horizon Discovery測序標(biāo)準(zhǔn)品,Biomatik公司Elisa試劑盒,Nanocs PEG修飾劑,Spectrumlabs透析袋,Polysciences微球,JenaBioscience分子試劑,101BioLifespan Technologies硫酸葡聚糖ELISA試劑盒及抗體,Lee Biosolutions抗體,Emfret心血管研究抗體,Magtivio B.V.DNA提取磁珠,Biontex Laboratories轉(zhuǎn)染試劑,Canvax?細(xì)胞凋亡檢測試劑盒,Matriks Biotek?生物藥物檢測試劑盒,Immunostep流式細(xì)胞術(shù)抗體，F(xiàn)uller Laboratories感染性疾病檢測試劑盒,SICGEN抗體,biotechrabbit酶及抗體等。